what is coloum chromatography?

Question

what is coloum chromatography?

Answer 1

It is a form of partition chromatography in which a liquid phase flows down a column packed with a solid phase.

In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column and the mobile phase, a liquid, is added to the top and flows down through the column (by either gravity or external pressure). Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.

Answer 2

Another type of column chromatography is called HPLC (for High Performance Liquid Chromatography). The column (packed with the solid phase) is shorter, smaller and usually horizontal. The mobile liquid phase is pumped through the column. A detector (usually an ultraviolet absorbtion type) is used to measure the compounds being separated.